In a multi-disciplinary collaborative effort between the van der Heden van Noort laboratory at the Dept. of Cell and Chemical Biology in the Leiden University Medical Center and the Ahel laboratory at University of Oxford researchers Max Kloet, Chatrin Chatrin and Rishov Mukhopadhyay and their teams prepared an ubiquitinated-ADPribose probe and identified a family of RNF E3-ligases to interact with it using proteomics.
Biochemical and biophysical studies on one of these (RNF114) revealed how this interaction takes place and what the minimal requirements are for this tight interaction that is crucial for recruiting RNF114 to sites of induced DNA-damage.
The paper was published in Nature Communications, check out the full story here.
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