We are using DNA to determine structure-function relationships between antigen nanopatterns and immune system activation, and developing methods to exploit this activation. We combine this with state-of-the-art phase-plate electron cryo-tomography to solve various structures of our immune system. We are also working to combine the burgeoning fields of super-resolution light microscopy with cryoEM to achieve high-accuracy localization of tagged proteins within samples prepared for cryoEM. This will allow us to perform structural biology on individual proteins within cells.
About the Sharp lab
We use a combination of DNA nanotechnology, synthetic biology, super-resolution light microscopy and high-resolution cryoEM to investigate immune system activation