To investigate the epicardium we look into epithelial-to-mesenchymal transition (EMT). For this we use human primary material from which I isolate epicardial-derived cells. I make sure these cells are characterized and are reliable to work with. We use the epicardial cells for e.g. a high-throughput screen, or for transplantation in vivo. I have also worked with many other cell cultures models including a flow culture system. Besides this I’m also lab manager for an ML I and ML II lab. As a biotechnician I apply in vivo models for cardiac disease (heart attack and heart failure), and I perform and analyze ultrasounds to measure cardiac function.
I studied Biology and Medical Laboratory Research at the University of Applied Sciences in Leiden, the Netherlands. My first job as a technician was at the AMC in the laboratory for Experimental Oncology and Radiobiology. Here I worked on a project with (colon) cancer stem cells for which I mainly did the primary cell culture, in vivo work, and biobanking. Now I work at the LUMC in the lab of professor Goumans in the group of Anke Smits which revolves around the role of epicardium in endogenous cardiac repair.
Capturing colorectal cancer inter-tumor heterogeneity in
patient-derived xenograft (PDX) models
: Prasetyanti PR, van Hooff SR, van Herwaarden T, de Vries N, Kalloe K, Rodermond H, van Leersum R, de Jong JH, Franitza M, Nürnberg P, Todaro M, Stassi G, Medema JP.
Int J Cancer. 2018 Aug 27. doi: 10.1002/ijc.31767. [Epub ahead of print] PubMed PMID: 30151914.
and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart
Dronkers E, Moerkamp AT, van Herwaarden T, Goumans MJ, Smits AM.
J Vis Exp. 2018 Apr 24;(134). doi: 10.3791/57370. PubMed PMID: 29757271; PubMed Central PMCID: PMC6100756.
Counteracts Myocardial Fibrosis in Pressure Overload-Induced Heart Failure.
Verjans R, Peters T, Beaumont FJ, van Leeuwen R, van Herwaarden T, Verhesen W, Munts C, Bijnen M, Henkens M, Diez J, de Windt LJ, van Nieuwenhoven FA, van Bilsen M, Goumans MJ, Heymans S, González A, Schroen B.
2018 Feb;71(2):280-288. doi: 10.1161/HYPERTENSIONAHA.117.10094. Epub 2017 Dec 18. PubMed PMID: 29255073.
Groups: Cardiovascular Cell Biology