My interest lies within the molecular biology techniques which I use to create all kind of constructs to investigate all the topics that are being studied in the Neefjes Lab. The project I’m most involved in is the unraveling of the retrofusion process of Multi Vesicular Bodies. Currently we are optimizing cloning strategies in combination with the Crispr/Cas system to generate a range of endogenous fluorescent tagged cell lines.
In 1997 I graduated from the HLO Delft specialization Biochemistry. I started to work in the lab of John Collard at the division of Cell Biology at the Dutch Cancer Institute (NKI) to search for new genes involved in the metastasis of cancer under the supervision of Dr. F. Michiels. On the 1st of January 2000 I got a permanent position as a research technician in the Neefjes Lab. My main objectives are supporting the group with my molecular biology skills, generating new scientific tools and managing the lab.
An ER-Associated Pathway Defines Endosomal Architecture for Controlled Cargo Transport.
Jongsma ML, Berlin I, Wijdeven RH, Janssen L, Janssen GM, Garstka MA, Janssen H, Mensink M, van Veelen PA, Spaapen RM, Neefjes J.
Cell. 2016 Jun 30;166(1):152-66. doi: 10.1016/j.cell.2016.05.078. PMID:27368102
Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.
van der Kant R, Fish A, Janssen L, Janssen H, Krom S, Ho N, Brummelkamp T, Carette J, Rocha N, Neefjes J.
J Cell Sci. 2013 Aug 1;126(Pt 15):3462-74. doi: 10.1242/jcs.129270. Epub 2013 May 31. PMID:23729732
The Rab7 effector protein RILP controls lysosomal transport by inducing the recruitment of dynein-dynactin motors.
Jordens I1, Fernandez-Borja M, Marsman M, Dusseljee S, Janssen L, Calafat J, Janssen H, Wubbolts R, Neefjes J.