Our cells contain a total repertoire of about 22,000 different proteins, each with their own roles and features. Despite the current advancement in our knowledge of cellular functioning, the function of many of these proteins remains unknown. Using genetic screenings (such as Crispr-Cas9 and RNAi) in combination with proteomics and classical cell biological techniques I’m trying to assign functions to some of these unstudied proteins. The focus of these efforts is on genes that control the endocytic network and antigen presentation.
Currently I’m working on a de-ubiquitinating enzyme that regulates the integrity of cell-cell contact sites, as well as cell migration and differentiation.
I studied biotechnology at Wageningen University and performed a research internship at the University of California San Francisco in the lab of Abul Abbas. After my Masters I started my PhD in the lab of Jacques Neefjes at the NKI, now followed by a postdoctoral period in the same lab in Leiden. During my PhD I worked on a diverse range of projects, among which identifying mechanisms of chemoresistance to the anti-cancer drug doxorubicin, as well as more fundamental biological questions such as the regulation of the degradation of autophagosomes.
Chemical and genetic control of IFNγ-induced MHCII expression.
Wijdeven RH, van Luijn MM, Wierenga-Wolf AF, Akkermans JJ, van den Elsen PJ, Hintzen RQ, Neefjes
Embo Reports, 2018 Sep;19(9)
Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway.
Wijdeven RH, Janssen H, Nahidiazar L, Janssen L, Jalink K, Berlin I, Neefjes J.
Nat Commun. 2016 Jun 10;7
Genome-Wide Identification and Characterization of Novel Factors Conferring Resistance to Topoisomerase II Poisons in Cancer.
Wijdeven RH, Pang B, van der Zanden SY, Qiao X, Blomen V, Hoogstraat M, Lips EH, Janssen L, Wessels L, Brummelkamp TR, Neefjes J.
Cancer Res. 2015 Oct 1;75(19):4176-87