I am working in Alfred’s group as a Research Technician. In practice, I am helping out PhD students, Post-docs or Alfred with their research. I am mostly helping people with projects based on the cell cycle, and the role that SUMO has within the cell cycle. This can be by helping them with pull-downs, microscopy, in vitro assays, purifying proteins etc. I am also helping to keep everything going, by ordering reagents in time and preparing common stocks.
I studied Biomedical Sciences (Major Forensic Sciences) at Avans Hogeschool in Breda. During my studies I did an internship at the Erasmus MC, department of Cell Biology, in the group of Gert Jansen. During this internship I worked on identifying the mutation in gene(s) within six different Caenorhabditis elegans strains which showed a shortening of the sensory cilia. This was done by crossing the mutant strains with a wild-type strain that has another genetic background. After the crossing with another strain you need to select the worms based on their shortened cilia phenotype. In the end (after several crossings with another genetic background) you can use SNP-Mapping to see on which chromosome the mutant gene is located, and also on which location within that chromosome. When you have localized this, you can see which genes are in this region and whether they are mutated. After this internship I realized that I am more interested in biology instead of the crime scenes, so then I applied to Alfred’s group. Just after finishing my studies, I joined Alfred’s group to do research on SUMO.
SUMO targets the APC/C to regulate transition from metaphase to anaphase.
Eifler K, Cuijpers SAG, Willemstein E, Raaijmakers JA, El Atmioui D, Ovaa H, Medema RH, Vertegaal ACO.
Nat Commun. 2018 Mar doi: 10.1038/s41467-018-03486-4.
Converging Small Ubiquitin-like Modifier (SUMO) and Ubiquitin Signaling: Improved Methodology Identifies Co-modified Target Proteins.
Cuijpers SAG, Willemstein E, Vertegaal ACO.
Mol Cell Proteomics. 2017 Dec doi: 10.1074/mcp.TIR117.000152