During my master study in chemical biology, I joined to Ovaa lab to learn how to combine my organic chemistry knowledge with chemical biology techniques to study the ubiquitin system in vitro. I mainly create chemical tools to study ubiquitin and deubiquitinating enzymes (DUBs). My first project was the synthesis of FRET-based diubiquitin probes with which we quantified the linkage specificity of DUBs. During my PhD, I started off with the synthesis of fully synthetic linear diubiquitin, which has never been published before. I will continue to study the kinetics of DUBs in the presence of different ubiquitin linkages and synthesize novel activity based probes to study ubiquitin proteases.
I studied Molecular Science and Technology (BSc) and Chemistry (MSc) at the University of Leiden and obtained my MSc degree in June 2016, cum laude. In 2015, I joined the lab of Huib Ovaa at the NKI for my MSc internship where I developed synthetic FRET-based probes to study the activity and specificity of deubiquitinating enzymes. After a short break, which I filled with teaching Chemistry in high school, I started my PhD in the Ovaa group in April 2017, where I work on the development of ubiquitin tools to study the ubiquitin system.
Native chemical ligation at methionine bioisostere norleucine allows for N-terminal chemical protein ligation.
Xin, B.T.* ; van Tol, B.D.M.*, Ovaa, H. ; Geurink, P.P.;
Org Biomol Chem. 2018, 16, 6306-6315.
Molecular basis of Lys11-polyubiquitin specificity in the deubiquitinase Cezanne.
Mevissen, T.E.T.; Kulathu, Y.; Mulder M.P.C.; Geurink, P.P.; Maslen, S.L.; Gersch M.; Elliott, P.R.; Burke, J.E.; van Tol, B.D.M.; Akutsu, M.; Oualid, F.E.; Kawasaki, M.; Freund, S.M.V.; Ovaa, H.; Komander, D.;
Nature, 2016, 538(7625), 402-405.
Development of diubiquitin-based FRET probes to quantify ubiquitin linkage specificity of deubiquitinating enzymes.
Geurink, P.P.; van Tol, B.D.M.; van Dalen, D.; Brundel, P.J.; Mevissen, T.E.T.; Pruneda, J.N.; Elliott, P.R.; van Tilburg, G.B.; Komander, D.; Ovaa H.;
ChemBioChem. 2016, 17 (9), 816-820.