Postdoc
Msc. Dharjath Ahamed Shahul Hameed
Research:
I did my MSc in Biomolecular Sciences, specialized in cell biology. On joining the lab of Huib Ovaa, I was trained in the synthesis of Ubiquitin (Ub) proteins, short peptides and other simple unnatural aminoacids which were used in solid phase peptide synthesis. After my short spell of being a technician, working mainly on synthesis of diubiquitins (diUb), I started my PhD research on more challenging aspects of Ub chemistry. My research spans the areas of development of ubiquitin conjugates including ubiquitin-peptide conjugates and diubiquitins; isotopic-labeled diUb molecules; synthetic cell-permeable Ub conjugates and Ub-based probes for all class of deubiquitinases. In addition, I also work on the development of peptide-based inhibitors for deubiquitinases. My expertise is the combination of peptide synthesis and its application in cell biology, which facilitates a better understanding of ubiquitin biology.
Curriculum Vitae:
I did my B.Tech in Industrial Biotechnology from Anna University, India. After graduating as MSc in Biomolecular Sciences from Vrije University in 2010, I started working as a technician in the lab of Huib Ovaa at the Netherlands Cancer Institute, Amsterdam. I then continued my research as a PhD graduate student, specializing in peptide synthesis and development of probes and assay reagents. My thesis is about how Ub chemistry helps to better understand the biology of Ub. After 2 years of continuing my research as post-doctoral fellow I am now in the process of transitioning to industry.
Publications
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Development of Ubiquitin-Based Probe for Metalloprotease Deubiquitinases.
Hameed, D.S., A. Sapmaz, L. Burggraaff, A. Amore, C.J. Slingerland, G.J.P. van Westen, and H. Ovaa
Angew Chem Int Ed Engl, 2019. 58(41): p. 14477-14482.
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Enhanced Delivery of Synthetic Labelled Ubiquitin into Live Cells by Using Next-Generation Ub-TAT Conjugates.
Hameed DS, Sapmaz A, Gjonaj L, Merkx R, Ovaa H.
Chembiochem. 2018. DOI: 10.1002/cbic.201800649
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Diubiquitin-Based NMR Analysis: Interactions Between Lys6-Linked diUb and UBA Domain of UBXN1
Hameed, D.S., G.B.A. van Tilburg, R. Merkx, D. Flierman, H. Wienk, F. El Oualid, K. Hofmann, R. Boelens, and H. Ovaa
Front Chem, 2019. 7: 921.
