iPSC Hotel

LUMC iPSC Hotel

The LUMC iPSC Hotel offers the generation of research grade human induced pluripotent stem cells (iPSCs) from different tissue sources using state of the art reprogramming techniques. The service includes isolation of somatic cells, reprogramming into hiPSCs and basic characterization of pluripotency and is free of charge for LUMC researchers. Costs apply for external parties. Our facility also offers support for hiPSC-based project planning, provides validated protocols and control human iPSCs, and organizes training courses on an annual basis.

The iPSC Hotel was founded in 2010 as a joint initiative of the departments of ANA and CCB. Our initial goal was to provide LUMC researchers as well as external customers with research-grade, high quality human iPSCs. The iPSC core facility has evolved into an iPSC Hotel with many more services since. 

 Current services

  • The generation of high quality human iPSCs from primary material such as skin, peripheral blood, and urine using different reprogramming techniques.  

        This service includes:        

  • Isolation, expansion and banking of the primary cells by the Hotel.
  • A basic characterization of the generated iPSC lines for pluripotency using  flow cytometry and immunocytochemistry.                 
  • Cell line authentication with STR profiling

 Optional services available:     

  • Additional characterization (e.g. karyotyping, PluriTest, ScoreCard)
  • Provision of control iPSC lines generated in house.
  • Advice and support regarding iPSC project planning and assay development.
  • Supply of differentiation protocols developed or implemented in house.


Future services

Genetic modification (isogenic controls/ knockin): The LUMC hiPSC hotel is currently establishing protocols for CRISPR/Cas9-based gene editing for generation of isogenic control hiPSCs and introduction of mutations into hiPSC lines.

Rent a room: One of our goals is to facilitate the differentiation of human iPSC for LUMC researchers. The hotel will support researchers to differentiate their iPSC line of choice into the desired cell type(s)/organoids under our direct supervision in a tissue culture room of the Hotel.

Training

Once a year the LUMC iPSC Hotel hosts a workshop on pluripotent stem cells.


CONTACT

Harald Mikkers
E-mail: h.mikkers@lumc.nl
Tel: +31 (0) 71 526 9668

iPS Hotel Facility
Department of Cell and Chemical Biology (CCB)
Leiden University Medical Center (LUMC)



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