Dr. Abhishek Sinha

Research:

I am an experienced researcher having previously four years of research experience in Biochemistry, Cell Biology and Genetics. I am presently interested in uncovering new chemical inhibitors that works on cancer’s. Specifically, I am focused in breast cancer model’s and the effect of novel inhibitors have on key intracellular signaling pathways like TGFb, MAPK, Akt etc. Finding mechanisms of inhibition is crucial to my projects. I am also keenly interested to supervise new research projects that aim to uncover the role of diverse deubiquitinating enzymes in diverse cancer models.

Curriculum Vitae:

I am from India. I did my Ph.D from Department of Biological Sciences, Indian Institute of Science Education and Research-Kolkata (2008-2014), under the guidance of Prof. Srimonti Sarkar. My Ph.D work was focused on uncovering the structure and function of proteasome in parasitic protist Giardia. I also worked on projects related to Phosphoinositide kinetics and ER resident protein translocating channel of the parasite. My first Post Doctoral stint was with Dr. Elah Pick at Department of Biology and Environment in University of Haifa (2014-2018). There I worked extensively on Cullin E3 Ligases (CRL’s). My projects were focused mainly on the role played by redox homeostasis on the NEDDylation/deNEDDylation dynamics within cell. I also worked on project’s understanding the role of CRL’s in lipid homeostasis in yeasts. Presently in LUMC I am carrying out high throughput screens of a large number of compounds against diverse cancer model’s.

Awards:

CSIR Senior Research Fellowship 2012-2014 (During Ph.D)

VATAT-PBC Post Doctoral Research Fellowship 2014-2017

Publications:

Articles in Journals:

1. Abhishek Sinha, Sananda Mandal, Sumana Banerjee, Alok K. Sil, Sandipan Ganguly, Srimonti Sarkar. (2011) Identification and characterisation of a FYVE domain from early diverging eukaryote Giardia lamblia. Curr. Microbiol. 62:1179-1184

2. Abhishek Sinha, Shankari Prasad Datta, Atrayee Ray, Srimonti Sarkar (2015) A reduced VWA domain-containing proteasomal ubiquitin receptor of Giardia lamblia localizes to the flagellar pore region in microtubule-dependent manner. Parasit Vectors. 8: 120

3. Abhishek Sinha, Atrayee Ray, Sandipan Ganguly, Subhra Ghosh Dastidar, Srimonti Sarkar (2015) Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia. Biol Direct, 10: 56

4. Ananya Jana, Abhishek Sinha, Srimonti Sarkar (2017) Phosphoinositide binding profiles of PX domains of Giardia lamblia. Parasitol int. 66: 606-614

5. Laylan Bramasole#, Abhishek Sinha#, Sylvia Gurevich, Meytal Radzinski et al. (2019) Proteasome lid bridges mitochondrial stress with Cdc53/Cul1 Rubylation status. Redox Biol.20: 533-543 [#co-First authors]

6. Laylan Bramasole, Abhishek Sinha, Angela Cirigliano, Sylvia Gurevich et al. (2019)Rpn11 DUB activity precedes cullin1 deNEDDylation activity of the COP9 signalosome during the transition of S. cerevisiae cells into quiescence. Biomolecules.9:9

7. Abhishek Sinha, Ran Israeli, Angela Cirigliano, Shalev Gihaz et al. (2020) The COP9 Signalosome Mediates the Spt23 Regulated Fatty Acid Desaturation and Ergosterol Biosynthesis. FASEB J. DOI: 10.1096/fj.201902487R

Book Chapters:

1. Abhishek Sinha, Srimonti Sarkar. (2013) “Ubiquitin-Proteasome System- a target to control pathogenic protozoa” in the book entitled “Microbial pathogens and strategies forcombating them: science, technology and education”. Formatex research center. ISBN (13):978-84-939843-9-7

2. Abhishek Sinha. (2018) “Fluorescence dyes and their usefulness in Yeast Research” in the book entitled “Microscopy Science: Last Approaches on Educational Programs and Applied Research”. Formatex research center. ISBN(13): 978-84-947512-3-3

3. Abhishek Sinha and Elah Pick(2020) Fluorescent detection of diauxic shift in budding yeast using ROS specific dyes. {Accepted in Methods in Mol. Biol. (In Press)}